Aktivitas Antibakteri Dan Formulasi Krim Ekstrak Etanol Daun Teh-Tehan (Acalypha Siamensis) Terhadap Propionibacterium Acnes

Abstract

Acne is a disease caused by P. acne bacteria which can cause inflammation. Acne treatment can be done by reducing the number of P. acne colonies using antibiotic. Teh- tehan leaf contain compounds that are antibacterial such as flavonoid, phenol, steroid, alkaloid and tannin. The purpose of this study was to determine the antibacterial activity of extracts and formulations of cream teh-tehan leaf extract against P. acne bacteria. This research was conducted in the laboratory of STIKes Ibnu Sina Ajibarang. This type of research is an experimental study using the disc diffusion method (Kirby and Bauer test) with different concentrations. The concentrations of extract used were 1%, 2% and 3% with a negative control (DMSO) and a positive control (Mediklin Cream) as comparison. Physical evaluation of the preparation of teh-tehan leaf extract cream consisted of: organoleptic test, homogeneity test, pH test, spreadability test, cream type test, adhesion test and cycling test. The results of this study indicate that the teh-tehan leaf extract can be formulated as an active substance in cream preparation. The results of testing the antibacterial activity of teh-tehan leaf extract cream, it can inhibit the growth of P. acne bacteria with the best concentration of 3% concentration. The teh-tehan leaf extract formulated with concentrations of 2% (FII) and 3% (FIII) and has an average inhibition zone of 4.60 mm and 9.01 mm. Furthermore, the results of the physical evaluation of the preparation of the teh-tehan leaf extract cream showed instability in the organoleptic test where there was a change in the color of the cream after the cycling test.