Uji Aktivitas Antijamur Ekstrak Propolis dari Lebah Trigona Sp asal Balikpapan terhadap Candida Tropicalis
DOI:
https://doi.org/10.59841/an-najat.v3i4.3377Keywords:
Antifungal, Candida albicans, Candida tropicalis, Propolis, Trigona spAbstract
Fungal infections are common health problems in tropical regions, including Indonesia, because humid environmental conditions support the growth of these microorganisms. Candida tropicalis is the pathogenic fungal species most frequently found, both as the cause of superficial and systemic candidiasis. The increasing resistance to synthetic antifungal drugs, such as fluconazole and ketoconazole, creates the need for alternative therapies that are safer and more effective. Propolis, a resinous substance produced by Trigona bees from various plant sources, is known to contain flavonoids, phenolics, and terpenoids that have important biological activities, including antimicrobial and antifungal effects. This study aimed to evaluate the effectiveness of ethanol extract of propolis from Balikpapan against the growth of Candida tropicalis using the disk diffusion method. Phytochemical screening showed that the propolis extract contained alkaloids, flavonoids, phenolics, tannins, and terpenoids. The antifungal activity test demonstrated that extract concentrations of 80%, 90%, and 100% significantly inhibited fungal growth, with the largest inhibition zone observed at 100% concentration (27.93 mm). Statistical analysis using ANOVA confirmed significant differences among treatments. Therefore, it can be concluded that propolis extract from Trigona bees in Balikpapan has strong potential as a natural antifungal agent. These findings support the utilization of propolis as an alternative natural-based therapy for fungal infections and as a contribution to the development of natural pharmaceutical products.
References
CLSI. (2018). Method for Antifungal Disk Diffusion Susceptibility Testing of Yeasts. . . Clinical and Laboratory Standards Institute, 38(24), 1–31.
Hossain, R., Quispe, C., Khan, R. A., Saikat, A. S. M., Ray, P., Ongalbek, D., Yeskaliyeva, B., Jain, D., Smeriglio, A., Trombetta, D., Kiani, R., Kobarfard, F., Mojgani, N., Saffarian, P., Ayatollahi, S. A., Sarkar, C., Islam, M. T., Keriman, D., Uçar, A., … Cho, W. C. (2022). Propolis: An update on its chemistry and pharmacological applications. Chinese Medicine, 17(1). https://doi.org/10.1186/s13020-022-00651-2
Kemenkes RI. (2017). Farmakope Herbal Indonesia Edisi II. Jakarta : Kementerian Kesehatan RI.
Khafid, A., Wiraputra, M. D., & Putra, A. C. (2023). Uji Kualitatif Metabolit Sekunder pada Beberapa Tanamanyang Berkhasiat sebagai Obat Tradisional. Buletin Anatomi Dan Fisiologi, 8(1), 61–70.
Khairunnisa et al. (2020). Karakteristik Fitokimia dan Aktivitas Antioksidan Ekstrak Propolis Lebah Trigona Sp. Jurnal Industri Pertanian, 2(1), 124–129. http://jurnal.unpad.ac.id/justin
Moghim, H., Taghipour, S., Kheiri, S., Khabbazi, H., & Baradaran, A. (2021). Antifungal effects of iranian propolis extract and royal jelly against candida albicans in-vitro. International Journal of Preventive Medicine, 12(1), 1–5. https://doi.org/10.4103/ijpvm.ijpvm_420_18
Nubla, S., Adawiyah, R., Sahlan, M., Tugiran, M., Widyantari, N. M. C., & Harlim, C. (2024). Phytochemical analysis and antifungal activity propolis Lombok against Candida sp and Cryptococcus sp. Journal of Research in Pharmacy, 28(5), 1391–1399. https://doi.org/10.29228/jrp.817
Papp, Z., Bouchelaghem, S., Szekeres, A., Meszéna, R., Gyöngyi, Z., & Papp, G. (2021). The Scent of Antifungal Propolis. Sensors, 21(7), 2334–2342. https://doi.org/10.3390/s21072334
Pasupuleti, V. R., Sammugam, L., Ramesh, N., & Gan, S. H. (2017). Honey, Propolis, and Royal Jelly: A Comprehensive Review of Their Biological Actions and Health Benefits. Oxidative Medicine and Cellular Longevity, 20(17), 1–21. https://doi.org/10.1155/2017/1259510
Pazra, D. F., Multida, I., Nurlita, S., & Sari, M. (2023). Ekstrak Cacalincingan (Oxalis barrelieri L) Sebagai Antibakteri Terhadap Staphylococcus aureus dan Escherichia coli Penyebab Mastitis Sapi Perah. Jurnal Veteriner, 23(3), 360–370. https://doi.org/10.19087/jveteriner.2022.23.3.360
Pratami, D. K., Indrawati, T., Istikomah, I., Farida, S., Pujianto, P., & Sahlan, M. (2020). Antifungal activity of microcapsule propolis from Tetragonula spp. to Candida albicans. Communications in Science and Technology, 5(1), 16–21. https://doi.org/10.21924/cst.5.1.2020.178
Rivera-Yañez, C. R., Ruiz-Hurtado, P. A., Reyes-Reali, J., Mendoza-Ramos, M. I., Vargas-Díaz, M. E., Hernández-Sánchez, K. M., Pozo-Molina, G., Méndez-Catalá, C. F., García-Romo, G. S., Pedroza-González, A., Méndez-Cruz, A. R., Nieto-Yañez, O., & Rivera-Yañez, N. (2022). Antifungal Activity of Mexican Propolis on Clinical Isolates of Candida Species. Molecules, 27(17), 5651–5673. https://doi.org/10.3390/molecules27175651
Rizki, S. M., & Panjaitan, R. S. (2018). Efektivitas Antifungi dari Minyak Atsiri Kulit Batang Kayu Manis (Cinnamommum burmanni) terhadap Candida albicans. EduChemia (Jurnal Kimia Dan Pendidikan), 3(2), 172–183. https://doi.org/10.30870/educhemia.v3i2.4560
Rodiah, A. S., Fifendy, M., & Indriati, G. (2022). Uji Daya Hambat Ekstrak Daun Beringin (Ficus Benjamina L.) Terhadap Pertumbuhan Jamur Candida albicans secara in Vitro. Serambi Biologi, 7(4), 318–325.
Salomão, K., Pereira, P. R. S., Campos, L. C., Borba, C. M., Cabello, P. H., Marcucci, M. C., & de Castro, S. L. (2018). Brazilian Propolis: Correlation between Chemical Composition and Antimicrobial Activity. Evidence-Based Complementary and Alternative Medicine, 5(3), 317–324. https://doi.org/10.1093/ecam/nem058
Valerio, T.-F. K., Bonfim-Mendonça, P. S., Rosseto, H. C., Bruschi, M. L., Henriques, M., Negri, M., Silva, S., & Svidzinski, T. I. (2016). Propolis: A Potential Natural Product to Fight Candida Species Infections. Future Microbiology, 11(8), 1035–1046. https://doi.org/10.2217/fmb-2015-0016
Yarlina, V. P. (2020). Kajian Konsentrasi Etanol dan Metode Ekstraksi Propolis dari Lebah Jenis Trigona sp. Terhadap Aktivitas Antimikroba Bakteri Escherichia coli dan Beberapa Karakteristik Ekstrak. Jurnal Teknologi & Industri Hasil Pertanian, 25(1), 27. https://doi.org/10.23960/jtihp.v25i1.27-34
Yusika, D. A., Islam, I., & Sahlan, M. (2023). Analisis Kadar Polifenol Total dan Flavonoid Total Propolis Asal Tanah Laut dan Soppeng. Journal of Life Science and Technology, 1(1), 7–12.
Zahra, N. N., Muliasari, H., Andayani, Y., & Sudarma, I. M. (2021). Karakteristik Fisikokimia Ekstrak Madu Dan Propolis Trigona Sp. Asal Lombok Utara. Jurnal Agrotek Ummat, 8(1), 7–16. https://doi.org/10.31764/jau.v8i1.3826
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